Congo red staining kit

Amyloid deposits will stain red, while nuclei will stain blue. Amyloid stain kit used in the detection of amyloid in formalin fixed paraffin embedded ffpe tissue sections as well as frozen tissue. The congo red stain kit amyloid stain kit is intended for use in the histological visualization of amyloid in tissue sections. Congo red staining kit kit for the detection of amyloid acc. Does anyone have a working protocol for preparation of congo red solution and staininig mouse brain slice. The use of polarizing lenses is the essential technique for visualizing amyloid positive areas andor to. The results described here show that congo red binds to many native proteins and lacks secondary structure specificity. Demonstrate amyloid with thermo scientific richardallan scientific amyloid stain. Amyloid, bennhold congo red stain kit identifies the extraneous protein deposits in amyloidosis. Congo red amyloid stain kit is used for the detection of amyloid in formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. The ability to detect amyloid deposits in human tissue is the critical first step in making a diagnosis of amyloidosis. Summary and explanation the congo red stain kit is used to identify amyloid. The use of polarizing lenses is the essential technique for visualizing amyloid positive areas andor to confirm negativity.

Amyloid congo red stain kit 125ml avantik biogroup. The amyloid stain kit congo red is intended for use in the histological visualization of amyloid in tissue sections. Jan 05, 2020 congo red histological staining technique is the gold standard technique for the diagnosis of amyloidosis. Newcomer supply amyloid, puchtler congo red stain kit procedure is used in identifying the extraneous protein deposits in amyloidosis. This kit is suitable in lieu of and comparable to the alkaline congo red method, commonly used to stain for. Used in the detection of amyloid ffpe as well as frozen tissue sections. After that, dry the slide in room temperature and observe under light microscopy 100x magnification oil. Congo red stain kit highman from atom scientific stain. Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. It is used for the detection of amyloid on formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. Amyloid, elastin, keratin, eosinophils orange red nuclei blue. Does anyone have a working protocol for preparation of. Pdf evaluation of congo red staining kit to determine. Examination under a polarizing microscope results in green birefringence of amyloid.

For capsule staining i used over night bacteria culture and smear with 1% congo red. Complete kit to aid in the staining of tissue for amyloidosis. The main special stain used for amyloid detection is congo red. It can identify very tiny amounts if applied properly.

Common diseases for congo red stain include primary amyloidosis, al. The mastertech congo red amyloid kit requries no working solution preparation, making it faster and more convenient than similar kits or procedures. Amyloidosis can affect different organs in different people and it can be diagnosed using the congo red amyloid stain kit from american mastertech. Examination under a polarizing microscope results in apple green birefringence of the amyloid.

Alkaline congo red solution serves as the primary stain, producing pink to red amyloid deposits with blue nuclei counterstained by hematoxylin. Congo red certified biological stain, fisher chemical. View or download the congo red staining kit msds material safety data sheet or sds for 101641 from merck. Finally, immunohistochemical staining can be used to confirm amyloid tissue. Alkaline congo red amyloid stain kit stains for amyloid deposits in less than 15 minutes. Amyloid stain kit congo red amyloid stain kit congo red amyloid stain kit congo red sku. This would explain the falsepositive results obtained in tissue sections with cytoskeletal proteins that are stable under the conditions used for congo red staining in tissue sections.

The addition of the alkaline salt solution in the puchtler modification of the bennhold method reduces background birefringence. This applegreen birefringence is considered pathognomonic for amyloid fibril deposits. All stains will be handled as stain and return unless a consultation is requested. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This amyloid, puchtler congo red stain kit procedure, is used for the demonstration of amyloid in tissues. Principle congo red dye forms nonpolar hydrogen bonds with amyloid and red to apple green birefringence occurs when viewed by polarized light due to alignment of dye molecules on the lineraly arranged amyloid fibrils. This attachment is achieved by the nonpolar hydrogen bonds formed between the congo red and amyloid deposit. Vitroview congo red amyloid stain kit vitrovivo biotech.

It is used to evaluate the presence and extent of amyloidosis in different organs. Amyloid is an abnormal protein product that can be found in various pathologic conditions. This method uses a solution of congo red which has a greater affinity for amyloid. Congo red stain kit, artisan special stain agilent. Negative stain kit set of 3 30ml microscope stains congo red, hydrochloric acid, formalin nigrosin solution. This stain kit is used for the detection of amyloid in tissue sections. Alamyloidosis presenting with negative congo red staining in. Congo red staining is based on the formation of hydrogen bridge bonds with the carbohydrate component of the substrate. Amyloid bennhold congo red stain kit newcomer supply. Control tissue is alzheimers or other known amyloidosis. This kit is commonly used to stain for primary, secondary, myelomaassociated, or tumorassociated amyloidosis. Since this color change is an approximate inverse of that of litmus, it can be used with litmus paper in a simple parlor trick. This procedure can identify minute amounts of amyloid.

Amyloid, bennhold congo red stain kit uses a procedure that is very useful in identifying the extraneous protein deposits in amyloidosis. Stain amyloid in tissue sections with congo red stain kit amyloid stain ab150663. Mar 10, 2020 as congo red only staining the polysaccharide which is through ica pathway, it can be inferred these two samples, which are congo red negative, are forming biofilm by protein no ica pathway or. This kit is suitable in lieu of and comparable to the alkaline congo red method, commonly used to stain for primary, secondary, myelomaassociated, or tumorassociated amyloidosis. Congo red staining kit is intended for use as a qualitative histologic stain to selectively demonstrate amyloid in formalinfixed, paraffinembedded tissue. The congo red staining kit is used for the detection of amyloid, which is relevant for several illnesses such as alzheimers, creutzfeldtjakob disease, bovine spongiform encephalopathy, myopathies and angiopathies. Amyloid is an abnormal protein product that can be. Congo red amyloid stain kit only from american mastertech. Alkaline congo red solution will stain amyloid orangeto red and display apple.

Congo red certified biological stain, fisher chemical click to view available options quantity. To request a consultation, submit the pathology report, all associated case materials clinical history, blocks, slides, etc. Congo red stain kit is used to identify amyloid in tissue sections on artisan link and artisan link pro staining systems. The congo red stain kit is used to identify amyloid. The staining kit contains readytouse congo red solution and koh differentiation solution. Alkaline congo red amyloid stain kit only from american. The reason for this is that the congo red dye used in the stain is highly selective for amyloid deposits, which can lead to amyloidosis and also alzheimers. Staining is intensified by pretreatment of tissue with alkaline sodium chloride. While there are several ways to stain for amyloid, congo red staining is considered the most specific technique in demonstrating amyloid in a tissue. Amyloid protein is detected in tissue with congo red, a metachromatic stain. Stains for amyloid deposits in just over 60 minutes. Lemon, white, red and orange, chili, congo black, productive, from usa.

Staining kits for histology ivdoem materials and reagents. The congo red staining is routinely used to visualize amyloid structures with the method acc. This method uses salts to reduce the background electrochemical staining and enhances the bonding between congo red dye and amyloid. Congo red is an organic compound used for staining amyloid in tissue sections.

Register today to view list prices and apply for an account to purchase online. Amyloid stain kit used in the detection of amyloid ffpe as well as frozen tissue sections cut at 10 microns. Overview the amyloid stain congo red is intended for use in the histological visualization of amyloid in tissue sections. Alkaline congo red solution will stain amyloid orangeto red and display applegreen birefringence when examined with polarizing microscope. Dec 09, 2012 congo red stain applied to the tissue gives the amyloid protein a salmonpink color and placed under polarized light the amyloid proteins have an applegreen birefringence. Amyloid, puchtler congo red stain kit newcomer supply. This b ennhold s congo red stain is used for the detection of amyloid on formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. Negative stain kit set of 3 30ml microscope stains congo. Modified highmans congo red staining protocol for amyloid. Avantor is a trusted global partner to customers and suppliers in the life science, advanced technology and applied materials industries. Preeclampsia is characterised by the increased excretion of misfolding protein, attracted by a diazo dye denominated congo red. We operate in more than 30 countries and deliver an extensive portfolio of mission critical products, services and solutions.